Re: [DIYbio] Miniprep without EDTA

Awesome detail, and thanks for the license Cory!

Cory Tobin <cory.tobin@gmail.com> wrote:

>> Two things about vinegar:
>> One, 'distilled' vinegar isn't itself distilled: it means,
>> rather, that it was fermented using a very rapid fermentation
>> process from distilled ethanol. So, there are fair odds that
>> it's not a pure or sterile product. However, in a miniprep
>> that's probably not a big issue.
>
>Yeah, the only reason I said to use distilled white vinegar is because
>I tried a bunch of different vinegars and this type worked the best.
>In terms of maximizing 260:280, they all worked relatively well, but
>the distilled white also maximized the 260:230 which means there was
>less carbohydrate contamination.
>
>Pure acetic acid was the best (slightly higher DNA concentration and
>less carbohydrate) but I wouldn't recommend anyone use that without
>proper protective gear and ventilation. - preferably a fume hood.
>
>
>> License?
>
>Just added a CC BY 3.0 license on the page.
>
>
>-cory
>
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