Re: [DIYbio] Re: DIY electroporation protocol

> Yes pGLO is amp resistant, not sure what you mean by satellite colonies

The gene that confers amp/carb resistance is beta lactamase. That
protein gets secreted into the media surrounding the amp/carb
resistant colony and breaks down the antibiotic in the surrounding
region. This means that non-resistant colonies can form in a ring
around the resistant colony. This usually happens when you let the
plate incubate for more than a day.

-cory

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