Ahh, to be clear there were only two colonies on 12 non-control plates, and those two were at edge so they might just be contamination. I doubt it has anything to do with satellite colonies
On May 9, 2012 7:23 PM, "Cory Tobin" <cory.tobin@gmail.com> wrote:
-- > Yes pGLO is amp resistant, not sure what you mean by satellite colonies
The gene that confers amp/carb resistance is beta lactamase. That
protein gets secreted into the media surrounding the amp/carb
resistant colony and breaks down the antibiotic in the surrounding
region. This means that non-resistant colonies can form in a ring
around the resistant colony. This usually happens when you let the
plate incubate for more than a day.
-cory
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