Re: [DIYbio] Re: DIY electroporation protocol

On 05/09/2012 04:31 PM, Nathan McCorkle wrote:
> I think the whole process was too dilute cell-wise.

The 1 cm drag was starting point, then instead of streak reduction
of concentration you mention fresh toothpicks. 6 times.

Does a fresh toothpick have any cells on it, or sterile?
Not understanding all of it.

6 shaking dilutions with the *same* toothpick would be a huge dilution factor --
PPM I'd guess.

John

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