Once again I came over the term biobrick...
I know what it should mean (Promotor is one brick, gene another, origin of replication , etc.)
But when you put them together, why are you sure that they will have the right place (1-2-3-4) instead of sticking together anyhow (in a 4-2-1-3 manner) ??
Do they have speciel restriction sites?? That do not attach to itself?
If I understood correctly, one can assemble plasmids (or even entire genoms) using this technique. And if the promoter doesn't work, just take aonther biobrick-promotor. And then it may work.
Maybe a stupid question for one who is familiar with it...
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