Hi there,
The problem is that sometimes the procedure works and we get the correct bands showing up in electrophoresis, while other times we get no bands at all. We always follow the same general procedure, sometimes varying the quantities of reagents and PCR times to try and get it to work. Repeating the exact procedure from the times it did work doesn't lead to it working again.
As the extraction process is the biggest variable we assume this is at fault. At the moment we extract it from cheek cells by scraping with a pipette tip. I've linked to the project page if anyone is interested, all info is there.
Cheers
Will
On Thursday, 28 June 2012 13:59:39 UTC+1, phDIY wrote:
Can you elaborate on your problems?
On Wednesday, June 27, 2012 6:12:10 PM UTC-5, TRolandB wrote:Hi everyone, our group (London hackspace) have been doing some genetic tests (sex typing) recently, with PCR and electrophoresis. We're getting very inconsistent results, and think our extraction process is at fault, so we want to try some similar techniques but with DNA from foodstuffs, so we can be sure of getting the extraction right.Does anyone have any suggestions of tests you can do that involve PCR and electrophoresis of this type?Will
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