Thought it would be best to start a new thread for this.
I had the light producing genes (Lux ABCDE) rewritten by a program to adapt it to Arabidopsis (Codon Bias).
Now I have to add promoter(s), RBS and terminator.
According to this http://bccm.belspo.be/db/lmbp_plasmid_details.php?NM=pJE202 , in the native plasmid there is only one promoter "Vibrio fischeri luxICDABEG operon promoter".
Translating this into plant genes, I just need one promotor for all the genes?? (Virus-Plant promoter, because of strong expression)
When I attach the promoter sequence to the gene segence, does there have to be sapce left or just add it like ATGTC + AAAA = ATGTCAAAA ??
I think this would be the best promoter available: CLCuMV C1. Does anyone know where to get the sequence from??
thx
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