Re: [DIYbio] Bio Bricks

Have a look at this page http://partsregistry.org/Assembly:Standard_assembly

If you look at the final product shown in the picture you will see
that the restriction sites in between parts get destroyed while the
two pairs of sites on either end of the combined part still exist. So
in any BioBrick compatible plasmid you only have 4 restriction sites,
no matter how parts you have combined into one. And the two ends of
the parts are different so you can specifically target which end you
want your insert to go to.


> If I understood correctly, one can assemble plasmids
> (or even entire genoms) using this technique. And if
> the promoter doesn't work, just take aonther
> biobrick-promotor. And then it may work.

Sort of. Once you assemble parts together there is no way to go back
and remove one part and replace it with another. You would need to
keep the all the plasmids you made up to that point, go back and find
the plasmid just before you added the part you want to swap, and use a
different part instead. Then rebuild the rest of the assembly. I'm
not sure I explained that clearly, if it is confusing let me know.

Since the BioBricks assembly doesn't let you swap out
already-assembled parts, the way people usually go about building
something big is they assemble a bunch of smaller components first,
test the small components, then start merging smaller components into
larger and larger ones until the whole thing is assembled. If you
build the whole thing at once and something doesn't work properly you
would need to rebuild the entire thing from the beginning.

-cory

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