Re: [DIYbio] How is Agrobacterium Tumefaciens grown? Is it possible to find in soil?

I hope you're not suggesting importing a _wild_ strain of agrobacterium into the US? That'd be morally and legally a 'bad thing'.

Barra Darcy <baradarcy@gmail.com> wrote:

>hmmm...
>I live in Ireland but would be going over to Indiana in August, I don't
>
>know if that's any help...it'd leave all the importation ('smuggling')
>up
>to me. I could send you the address if you have a e-mail?
>Is it only found over there or is this world-wide anyway?
>
>On Monday, June 18, 2012 7:09:52 AM UTC+1, cory....@gmail.com wrote:
>>
>> > This wonderful 'stuff' seems to be the 'gold dust' for doing
>genetics
>> with:)
>> > Does anybody know anything about it? Like where to find it, what
>> conditions
>> > and so on it likes.....How to encourage it?
>>
>> You can actually grow it on LB just like E. coli, although if you put
>
>> it in a 37C incubator it will die. It grows best on LB at 27C.
>>
>>
>> > Would love to know, even have somebody mail me a sample...maybe:)
>>
>> If you're in California then you're in luck. I can send you a sample
>
>> no problem. If you're in the US but outside California, get a permit
>
>> for interstate transportation of plant pathogens here:
>>
>>
>http://www.aphis.usda.gov/plant_health/permits/organism/plant_pathogens.shtml
>
>> and I'll send a sample. If you're outside the US, check the
>> regulations in your country and get the proper permits.
>>
>> Summary: transporting plant pathogens across political boundaries is
>
>> complicated, even if it's a "disarmed" strain like GV3101. Your best
>
>> bet is to find a plant genetics lab at a university near you and ask
>> the professor for a sample.
>>
>>
>> -cory
>>
>
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