My NaOH 'powder' is in a bad container, and now it has sucked water.... Now it's a very pure NaOH solution...
Have to get some new.
BUT: I succeded in getting a professional miniprep kit. I'd like to use it now.
I recently got a Dremelfuge from a friend who built his own 3D printer. As an inauguration present ;)
1) Will it be spinning fast enough to do a miniprep (Get most of the bacteria out of soultion)?? I once tried it, but it gave very little yield. But maybe, the lb marmelade jar was not full of of bacteria (althought they didn't glow anymore, so lysis?)
2) In which container shall I breed the 3-5 mL LB solution for the bacteria??
They will need oxygen, so above the tube shall be space. I have e.g. those https://encrypted-tbn0.google.com/images?q=tbn:ANd9GcQ8Q34n7YkKUL1gx99j4OL3efA0_NN9AgO2BHv28QgmxIfdT0w8 (the right one, 15 mL)
Is that sufficient?
3) Do they need 37°C and shaking? I don't have a device that heats or shakes them. But I have a hacked play-station controller which shakes when I apply a voltage. I could tape it to the controller and let it shake (never tested it for more than 1/2 an hour, but I'm confiden't it won't blow up)
How will I know they are ready for being harvested?
4) Shall I get some ampicillin for that? When they grow at 37°C, they will grow faster thus losing plasmids more poften. When I keep them at room temperature, they will lose much less plasmids I read. The yield may decrease, but will it be significantly?
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment