gel looks good actually, clear and crisp bands in the ladder is a good sign. The problem is in the PCR reaction, was this for a class or done at home or in a community lab (I suspect class because the thermal printout you have). If it's a class, I suspect the primers are good, unless they're untested prior to this course being taught. If the primers are good, then your DNA wasn't concentrated enough or just wasn't present. If you had chromosomal DNA but it was contaminated with nuclease, you'd see smears down the gel (fragments of all sizes).
The following is my 1% PCR gel run.
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As you can see the products have strangely run all the way until the end. The products also look to be further than the dye looks to be on the gel itself. I'm relatively new to PCR and not completely sure how to trouble shoot this. The first row is a 1 kb ladder and the third row is a control. The expected product should have had a product length of 611. I ran the PCR at 4 annealing temperatures 5 C below the Tm. Does anyone know where I should start in determining what may have gone wrong with my PCR/gel run?
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Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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