On Fri, Jun 29, 2012 at 11:20 AM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
> Those fuzzy bands at the bottom, if they are not loading dye, are 'primer dimers'. You get them when your primers are able to prime one another or self-prime, causing lots of wasted primer and 'distracting' the the reaction from the real product.
>
I agree. Primer dimers
Is the third row control without any primers? The control I run uses
all reagents including primers but not the template.
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Re: [DIYbio] Odd gel run following PCR
9:24 AM |
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