Re: [DIYbio] Open Gelbox/Transilluminator Source Files

On 06/12/2012 11:01 AM, Zebedeeboy wrote:
> I think miniaturisation is def the way to go as you suggest. Avoiding too much edge effect is going to be the key

By that do you mean the thinness of the starting point well where you
add your sample? So as to not yield a blurry group of same sized molecules,
but rather a sharp and discernable one?

We've seen some cartridge designs where they have a plastic funnel slot in the top for adding your sample,
but no control on how much. There seems to be a skill factor in your results -- too much
sample and get a blurry set of lines likely. A thin well made by a comb shape in the gel
seems best to me from a 3D fields and movement point of view. Then don't overfill it.

Does electrophoresis gel ever need to be sterile? how long will it keep at what temps? -- the cartridge
folks may be providing sterile, or with antibiotic preservative to keep from growing mold..

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