More details about plans for putting it in a vector and sequences will be avalible soon, but mostly I am looking for people who want to collaborate at bosslab. Two reasons:
1. make cool stuff for fun. It is easy to PCR genes out of plasmids, given that you have the right primers. Primers can even be used to attach new sticky ends to a sequence- lets say you have hindIII-RBS-pigment-stop-EcoR1, you can make SacI-RBS and stop-XhoI primers and do as you please with the DNA.
2. equipment shakedown @ bosslab
3. show people how to steal genes from the environment. Your floor is littered with thousands and thousands of bacteria, and each bacteria is full of DNA. Surely there must be something interesting down there...
On Mon, Jun 4, 2012 at 1:02 PM, Srdjan Gavrilovic <sasvimlogicno@gmail.com> wrote:
On Monday, June 4, 2012 5:02:29 PM UTC+2, Alex Hoekstra wrote:I'm game, of course, but you did sort of skip the "Where" aspect of the group project. Having online protocols so that folk from around the world can follow along on their own is nice, and of course some contributions can be made through decentralized chatting, but a lot of this work calls for in-person collaboration.
No, I did not miss "Where" part.
My point was to coordinate and try something new together. Reinventing wheel will not take us far.
Cloning might be done one day and colonies could be screened next day. After that plasmid can be isolated and blotted onto a peace of paper and sent to someone else to improve it (eg to add MCS) and there you have it a plasmid that rocks and can be distributed all through the community.
For this to work we have to find a way to do it in such manner that a product of one step can be used for the next.
I know that I'm not able to reach there for lab work but I might be the one to take it up after and I just want to see if we can organise it or should we do things everyone for our self and just keep talking about organisation.--To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/jX-W_0-qVRsJ.
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