Yeah, but those don't have self-excising marker genes.
And I reguard it as highly unethical to play around with chromosomal integration of antibiotic resistances, because if one bug escapes (and in diy bio this may hypothetically happen), resistant (really resistant, not just plasmids they lose again without selective pressure!) germs will spread .... What if an anthrax bacterium (relative to b.subtillis, so gene fits) takes up the DNA and gets resistant?
Thanks for the link regarding promotor in gram + and - ;)
2012/7/16 Nathan McCorkle <nmz787@gmail.com>
On Fri, Jul 13, 2012 at 6:43 AM, Mega <masterstorm123@gmail.com> wrote:a quick google of "subtilis homologous vector integration" comes up
> Hallo,
>
> Has anyone heard of a plasmid for integration in the chromosome of
> b.subtilis?
with loads of answers
www.bgsc.org/Catpart4.pdf
www.bgsc.org/NewProducts/Middleton.pdf
and something a little closer to home:
http://partsregistry.org/Bacillus_subtilis
"Integration plasmids is the most used technique by specialist in B.
subtilis. They are E. Coli plasmids with two homologous sequence with
two fragments of a chromosomal gene of B. Subtilis, and a resistance
cassette inserted in between. If your brick is cloned in between these
two homologous region, the vector act as a shuttle that integrate your
construct into the chromosome."
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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