Re: [DIYbio] Bacillus Subtilis integration vector

On Mon, Jul 16, 2012 at 5:01 PM, Andreas Sturm <masterstorm123@gmail.com> wrote:
> Yeah, but those don't have self-excising marker genes.

I don't think there is any system that is self-excising in use today
in the synbio world, it could probably be made using a stripped down
transposon type system though.

That said, if you integrate a marker, it doesn't have to be antibiotic
resistance. Even if it was, B. subtilis 168 is a lab strain, so it's
not likely to survive outside the lab. And it's just as easy to remove
the resistance yourself once you get your gene of interest in the
genome, you just knock it out using the same integration vector
technique.

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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