Ah ok, with this Cre-Lox System you would rely on that the amp reistance gets sorted out. Or would you reccomend inserting a counter marker (makes it non-auxotrophic, but where to get minimal-medium as a home genetic engineer)?
But it is kind of inevitable that the resistance is erased after some time, I think?
Additionally. I was wrong. You shouldn't take a B.Subtilis plasmid because it will replicate. Best would be something like pUC18, you amplify it in E.Coli and then miniprep -> B.Subtilis. If one subtilis can propagate, it has a copy of the gene in its chromosome.
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