Re: [DIYbio] Re: Finally a chance to create a glowing plant?

Yeah, but it affects the oxygen uptake which was the ''source idea''.



Am Donnerstag, 19. Juli 2012 02:17:05 UTC+2 schrieb Recovering Salesman:

I think you've misunderstood my use of "low pressure" - That's referring to the PSI of the pump and sprayer array compared to a "high pressure" system that uses a highly pressurized atomizer to turn the nutrient solution into such a fine mist that it passes through the outer membrane of the plant without actually wetting it.

You're talking about atmospheric pressure I believe(?), which is not something I've addressed.

Adam B. Levine
http://MindToMatter.org

On Wed, Jul 18, 2012 at 11:11 AM, Andreas Sturm <masterstorm123@gmail.com> wrote:

I read something from NASA regarding this:

Under low pressure, the plant thinks it loses too much water and gets stressed. (Mars base will have significantly lower pressure inside)

On the other hand, more pressure makes a contrary effect.



A willow is a good plant because it is really into water. It grows best near rivers, it's just like weeds...

2012/7/18 Adam Levine <adamlevinemobile@gmail.com>

Any ideas on what kind of plant to try this with?    If oxygen is a concern, I'd suggest using a low pressure aeroponics system - You can build one for about $80 at a hardware store.    

Plants grown in this method do not use a substrate, rather being grown in little net pots that let their roots hang in dark air below.  Under low pressure aeroponics, you basically have a recirculating nutrient solution stored in a reservoir.  A variable timer (one minute on, several minutes off) controls a pump in the solution which when activated forces the solution through a PVC sprayer assembly, comprehensively wetting the roots of the plant(s).   The pump turns off, the sprayed solution drains back into the reservoir.

This is ideal for many types of plants because it keeps the roots comprehensively wetted, but gives them regular and frequent "drying times' that encourage root growth as well as this whole type of system providing very granular control over the conditions your plant is subjected to.  

This could be done indoors or outdoors, but if you wanted to control stomata opening/closing you could do that internally with a controlled humidifier.

Adam B. Levine
http://MindToMatter.org

On Wed, Jul 18, 2012 at 7:00 AM, Cathal Garvey <cathalgarvey@gmail.com> wrote:

Do remember that bacterial bioluminescence and firefly bioluminescence,
and most other systems besides, are prime examples of _convergent
evolution_. They mostly bear no actual homology to one another.

That caveat shared however, you are right nonetheless: Bacterial
bioluminescence also requires and consumes oxygen. In plants, this
probably means you'll see best results under warm conditions with plenty
of water, which will encourage leaf stomata to open and allow free gas
exchange. You may find that plants in less ideal conditions will suffer
from oxygen limitation at night-time if they have to keep their stomata
closed to conserve water, and this might affect growth or survival.

Worth remembering when people raise the spectre of wild escapee plants,
I guess; there's no real advantage, and significant potential
disadvantages to bioluminescent plants in the wild.

On 16/07/12 17:49, Gavin Scott wrote:
> Since, as I understand it, the luciferin-luciferase reaction requires
> oxygen (I just read that fireflies glow by producing nitric oxide to
> temporarily prevent the mitochondria from consuming all the oxygen), might
> you not end up with a situation where your plants glow, but only in direct
> sunlight where photosynthesis produces a surplus of O2?
>
> G.
>


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