Re: [DIYbio] Re: Free plasmids for amateur biologists.

Maybe I'm misunderstanding something here but, I would be a little circumspect about using these plasmids in your home biology experiments. The plasmids encode surface proteins from the Hepatitis B virus. Since the genes are under control of a mammalian promoter (CMV) you are unlikely to get expression of the protein in E. coli but why mess with it. I think you should avoid working with pathogens or parts of pathogens until you are sure of what your doing, have a good reason for doing so, and have assessed the risks.  

 

 If all your interested in is learning how to do transformations then use something like pUC-19 or pGFP, or something else inocuous. I'd be happy to send you some (if I can). Contact me off list and let me know where you live and I'll see if I can send some plasmid without jumping through a bunch of red tape.

 

Actually I'll make this offer to anybody on the list-I would rather see people who want to learn this technology work with something that is safe rather then take a chance with something that could cause some problems and could raise some eyebrows if it was found that you are working with Hepatitis genes. It's a perception issue.

 

On Saturday, June 30, 2012 7:03:47 AM UTC-4, Dorif wrote:

Hello! I have found the way to get some free plasmids with map and work protocol. The Aldevron company distributes free samples of pRc/CMV-Hbs(S) and pCMVHB-S2.S plasmids. Both are based on pBlueScript plasmid and contain Ampicilline resistance gene. Each sample contains 2-4 ug of plasmid DNA. Samples are delivered via postage service. Link to order samples: http://www.aldevron.com/products/dnas/plasmids/ 

I hope it'll be useful for somebody.

P.S.: sorry for my bad English.)

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