We were tossing around the idea of a DIY gene gun at BioCurious as well. Sure, helium and gold particles may be more efficient, but I bet a hacked bb gun and, say, magnetite particles may give at least some transformation as well.
Here's a great video on how to prepare DNA "bullets", and how to use the gene gun to transform a small selection of cells in live neuronal samples:
http://www.jove.com/index/details.stp?id=675
Patrik
On Sunday, August 26, 2012 3:15:53 AM UTC-7, Mega wrote:
Hi,--
I 'm wondering how one could build such a device for particle bombardement.
They usually use Helium, but why not Nitrogen or Carbon dioxide (widely available). I read they use low pressure, but what is low?
Does it have to be in a vakuum??? I can do this, but it will be expensive and difficult to build the vakuum-tight casing and buy the vakuum pump.
Can there be too much pressure (so the particles fly through the cells without leaving DNA)? Or can I configure it just using 'some pressure' ?
(I have to look up the laws of my country, but as you cannot harm anyone with this device, it shouldn't be an issue)
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