[DIYbio] Re: Looking for Chloroplast integration Vector

I guess I will have to make my own. If I understood correctly, it seems quite doable:


http://www.plantphysiol.org/content/145/4/1129.full
From here, I need to make primers for the described integration target.
Add restriction sites.
Then I  isolate some genetic material from chloroplasts (dirty) and put it into a PCR.

Make primers for KanamycinR.

Then fuse   flankingsite - KanR - Lux - flankingsite into some plasmid.  Ready (?)

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