Hi Guys,
I come from the marketing side of the biotech business. Currently I'm a biotech marketing consultant (http://www.insysx.com) after working for companies such as Invitrogen, BioTrove (Life Tech) and Affymetrix.
So time to give you the definitive answers to Jonathan's and Nathan's speculations above.....
First QuantaBio used to be the manufacturer of BioRad's real time PCR mixes ... including for the BioRad iQ machines. Hence the mix which is specific to that machine. Other manufacturers' machines (e.g. ABI, Qiagen) generally require a passive reference dye called ROX to even out volume differences from well to well in the PCR plate. So that's the ROX ingredient in the ROX mix. Low ROX is a particular low concentration ROX variant variant on that system for reasons i won't get into here.
The CT in PerfeCTa is actually really cheesy marketing (says the marketer!). In real time PCR / qPCR analysis the key measure is the PCR cycle where fluorescence of the amplified DNA exceeds the threshold (background). This is referred to as the Threshold Cycle or CT. In quantitative real time PCR, s difference of 3.3 cycles in CT represents a ten fold difference in the quantity of original target. E.g. if one sample has a CT of 10 and another sample has a CT of 13.3, the first sample has a ten fold higher abundance of the target.
Best,
Matt Lawes PhD MBA
Principal
InSysX LLC
On Friday, August 24, 2012 2:29:10 AM UTC-4, Nathan McCorkle wrote:
On Thu, Aug 23, 2012 at 10:16 PM, Jonathan Cline <jnc...@gmail.com> wrote:--
> Is it just me or does anyone else have trouble keeping up with these crazy
> marketing names like
> PerfeCTa® SYBR® Green SuperMix for iQ™"
> versus
>
> PerfeCTa® SYBR® Green SuperMix, Low ROX™
>
>
> (rolls eyes)
>
> They might as well assume I'm a biologist or something, ha. It's a part
> time job just keeping up with the latest kit names.. maybe even worse than
> comparing Cisco network equipment!
>
well the iQ is a type of qPCR machine, I dunno what ROX is... I
imagine the 'CT' in perfeCTa has something to do with them trying to
say it binds indiscriminately to GC or TA pairs. That could all just
be what they /want/ me to think though...
>
> Anyway, it will take me 6 mos to a year to use the samples I've got. Yet
> the max shelf life rating is "2 mos at +20C". The oldest I've used is about
> 1 year and didn't notice much of a difference, but I have low yields on
> experiments anyway. I've only seen one manufacturer publish a curve for
> "reliability vs shelf life", otherwise info is ad hoc depending on who's
> using what in the lab. Too bad this data isn't part of the normal product
> spec.
>
Also,
From here:
http://www.invitrogen.com/etc/medialib/en/filelibrary/pdf/ focus.Par.34900.File.dat/ Focus%20Voume%209%20Issue%203.
"Making effective use of cloned M-MLV reverse transcriptase"
"In addition, keeping three of the cloned M-MLV RT preparations which
were 10 to 24 months old at 4 C for 7 days had no apparent adverse
effect upon the ability of the preparations to synthesize 7.5 and 9.5
kb cDNA from the RNA Ladder (data not shown). These results indicate
that purified cloned M-MLV RT has a shelf-life at -20 C in excess of 2
years and is not adversely affected by exposure to 4 C during shipping
or manipulations in the laboratory"
>
>
> ## Jonathan Cline
> ## jcl...@ieee.org
> ## Mobile: +1-805-617-0223
> ########################
>
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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