Yeah I was wondering about unbound pigment as well giving false positives, so to speak. But if SYBR green only flouresces when bound to DNA, that solves that problem. Though I only read the short Wikipedia article so might not have the whole story. Wouldn't one also have to worry about the fact that when you are reading UV 260, you are getting a reading from each nitrogenous base, GTAC, so "all" of the DNA so to speak.
But when you are looking at a dye, one dye molecule might bind every 10bp or 50 bp or 100bp or whatever. So one absorbance unit of dye =/= one absorbance unit of DNA. Maybe I'm explaining my point poorly...
I suppose that could be solved with some standards of known DNA concentration though, and comparing it with the reading one would get from a DNA binding dye.
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