Another question ;)
What if I clone in the lux operon twice?
Saying: Promoter - 5' UTR - LuxCDABE-CDABE-KanamycR - 3'UTR
Will it be twice as bright??
It will then be ~ 20 kbp (or a bit smaller). Can you still amplify it in E.coli? Or is it too big to enter cells by heat shock?
And will it get inside the plant cells when Polyethylenglycol transformation of protoplasts or gene gun is applied?
Tx
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