Re: [DIYbio] Re: Cheaper OpenPCR

On Tue, Oct 2, 2012 at 9:41 AM, Phil <philgoetz@gmail.com> wrote:
> Why are people so obsessed with building PCR machines? Will someone explain
> the reason?
>

Because they're a core Molecular Bio equipment and still expensive for
many people. We're talking in dollars. Convert the OpenPCR cost to the
currency in South American, Asian, or African countries and you can
see how expensive it is. How about the curious teenager or a High
School teacher whose budget (or School's budget) won't let them buy
such an expensive machine? If my budget is $1000, how much is left for
the other lab equipment?

> I advise people with those skills to use them designing or building
> something DIYers need and can't get, like a backup power supply that will
> keep a small freezer and a small incubator running for three days, or a
> small, lightweight, laminar flow hood.
>
No idea about the back-up power but as to Laminar Flow (a must have in
any lab). Go to Wal-mart and get a 19 gallon (or less, not sure how
big these are) clear storage container. Cut up the side with a Dremel
to get the opening. Add a fan to the top to blow air in and out in
front of you. Don't forget to add a HEPA filter on the fan. I am
assuming you are not working with dangerous chemicals or bio-hazards.
The will prevent contamination of samples. Might cost you less than
$70, depending on the items you buy.

Lots of examples from fungus growers:
http://www.fungifun.org/English/Flowhood

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