Re: [DIYbio] Re: Cheaper OpenPCR

Very true. Do you know of any modern Peltier pcr machine that has a user serviceable or hackable Peltier plate? Those things tend to die in 4-5 yrs. my old giant pcr machine runs off of fridge coils and compressors so all i have to do is refill the coolant with prestone 150. Anyone have the same luck with other old pcr machines?

Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC

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On Oct 2, 2012, at 6:17 PM, Jeswin <phillyj101@gmail.com> wrote:

> On Tue, Oct 2, 2012 at 3:28 PM, Bryan Bishop <kanzure@gmail.com> wrote:
>> On Tue, Oct 2, 2012 at 2:24 PM, Sebastian S. Cocioba wrote:
>>>
>>> You could ask the ebay seller to run through the self test. Just write
>>> very clear step by step directions on how and to post pics of each test
>>> result.
>>
>>
>> They aren't going to buy plasmids/DNA/primers, run gels and post results.
>> That just seems highly unlikely to me. I am not sure if anything less than
>> testing some reactions would be enough, really..
> That's right. If the machine runs the protocol, we only know that the
> MCU is working. We have no idea if the heat block is working properly
> or the temperatures are displayed accurately. The machine could have
> moisture damage or a cracked heat-block, for all we know.
>
> $600 is a lot for a single instrument. The coffee cup PCR is a good
> one, at half the price. If we use an innovative alternative to
> machined heat-blocks, like Simon's suggestion, then the price is
> better. Maybe the OpenPCR folks should build a budget version, based
> on that?
>
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