Re: [DIYbio] Re: Meeting times

The idea of a real, "open" parts registry idea comes up every so often
here, and I think a lot of people would like to see it happen, myself
included. On the other hand, and I may be wrong here, I don't think
anyone on this list has a laboratory with the capacity to serve as the
starting point for it. That's not to say it is impossible, or
couldn't be made to happen, perhaps with multiple places starting as a
beginning point working on a few parts at a time. I think an attempt
was made to pick out a handful of parts that people might like to see
in the beginning, so as to get a starting attainable goal of 40 or 50
pieces.

The funny (and really cool) thing about plasmids and these DNA parts
is, once you have them, you theoretically have them forever. As long
as you can do PCR or minipreps you can build up a stock as big as
you'd like.

I spent a few days two months ago reading all about part assembly and
the vector's used, but couldn't really ever find anything on the
licensing or policy concerning using them outside of iGem sanctioned
events. Even on the parts registry main page, nothing is written
under the Privacy Policy of Disclaimer links found at the bottom.

That aside, you can get this BioBricks assembly kit from NEB
http://www.neb.com/nebecomm/products/productE0546.asp which gives you
all the needed restriction enzymes to link parts together as well as
the ligase. I had thought previously (and incorrectly) that it also
came with the main biobrick vector, which it does not.

There are multiple vectors to use for parts assembly though
http://partsregistry.org/Plasmid_backbones/BioBrick_friendly and
http://partsregistry.org/Plasmid_backbones/Assembly

so it's a matter of what suits your needs and what is available.
Getting a BioBrick assembly vector that we could all share could be a
great first step.

If we were to start gathering parts on our own, I believe it would
make sense to continue to use the assembly standards layed out by
iGem, such that in the future, we could submit parts of our own to the
registry, while still maintaining an outside "open" registry, as well
as sharing with iGem teams or using parts we got from iGem teams or
other sources, that all fit into the assembly standard.

http://www.jbioleng.org/content/2/1/5

There is an article to an old paper by Knight going over the design
and thought processes behind the standard assembly vectors, and is
interesting.

Also, there is a decent amount of information on the parts registry
about part design and assembly, as well as on OpenWetWare I believe.
I found the parts registry extremely difficult to read and navigate
around at first, but after some time spent, you manage to get the hang
of it and it has some valuable information.

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