On Thu, Nov 22, 2012 at 1:09 PM, Sung won Lim <4phlebas@gmail.com> wrote:
They might be... But in my experience protoplasts tend to be quite unmistakable in their feature.
I'm no expert, and I haven't ever made these with plant cells, but you should be looking for perfect spheres with green inside them. Google images of protoplast look pretty distinct compared to most of your images, I think you need to grind up your sample better, and gently centrifuge it after the digestion and look for floaters in the upper areas of the tube.
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