3 & 4 look more like a bubble rather than a protoplast, specially if you consider the refringency.and lack of greenish or any internal stuff
41 and 42 are clearly ghosts. 16 and 17 could be partially digested cells as they are not so "squared", but hard to tell without a good reference of the start material
There are a lot of debris and the free green small "pearls" in #16 I would say are chloroplasts
El viernes, 23 de noviembre de 2012 13:35:29 UTC+11, Nathan McCorkle escribió:
--On Thu, Nov 22, 2012 at 1:09 PM, Sung won Lim <4phl...@gmail.com> wrote:
They might be... But in my experience protoplasts tend to be quite unmistakable in their feature.
I'm no expert, and I haven't ever made these with plant cells, but you should be looking for perfect spheres with green inside them. Google images of protoplast look pretty distinct compared to most of your images, I think you need to grind up your sample better, and gently centrifuge it after the digestion and look for floaters in the upper areas of the tube.
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