Re: [DIYbio] Methodology help needed - how to measure nail light absorbance

Ok guys, thanks for all the interest you have shown here.

I'm not quite happy where the discussion is going.
I have figured that some just can not hold their couriosity where it belongs :)

Ok here is why i need to be able to investigate infected nails absorbance: (Just remember to give me proper credit when you recieve your Nobel Prize for this)
For some time now laser devices are used to eradicate fungi from under the nail plate as a minimally invasive procedure.
I'm not quite sure if the wavelength of 1064 nm which is the most commonly used to achieve this - is a proper wavelength.

I have did my part of research and believe me there is no data on nail absorbance in this context.

If I would find differences in absorbance of specific fungal strains and healthy nail then this data would be useful in saying which wavelength is apropriate to hit just the fungus!

Ok - now there is no secrets

I have just one personal regard - Josiah I appreciate you are concerned that everyone does proper science - but here is the thing - there is no one recepie for science as this is the most abstract and innovative field of life !
So stop paternalising your peers!
You did not give any relavant information in this discussion apart from the fact that PCR is the most accurae in recognising fungal species.

This is not the problem I asked to give me some pointers on.

Maybe the new info will help you to figure some other ways to figure out how we can measure NAIL light band ABSORBANCE. I DO NOT CARE ABOUT THE FUNGUS SPECIES AT THIS MOMENT.

Also let's cut the talk on how to conduct science - as this does not get us anywhere.

W dniu piątek, 23 listopada 2012 00:19:50 UTC+1 użytkownik Nathan McCorkle napisał:
> I actually didn't say to use just PCR, I said PCR for the rRNA then sequence. I agree with Simon that currently and in the near future this isn't quick or cheap, but I don't see the OP saying they wanted a field instrument... they simply said they were a medical student interested in this stuff. There's a good chance we could just tell them to find a good on-campus spectrometer, UV/VIS IR etc...
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> On Thu, Nov 22, 2012 at 3:08 PM, Josiah Zayner <josiah...@gmail.com> wrote:
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> he simple question of what molecule one suggests would cause the difference in scattering I don't see a point in doing the experiment.
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> well you would be looking at trillions of molecules probably, but there is a chance of discoloration with the fungus vs host-only, especially if you can get some signal in the UV.
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> --
> -Nathan

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