It's not just a matter of a couple of point mutations to make the sequence non-identical. Gene patents can claim pretty much any level of sequence homology - and they frequently do, essentially leaving it up to court challenges to decide what level of homology is justified.
Patrik
On Thursday, November 22, 2012 1:36:35 PM UTC-8, Sebastian wrote:
Would it be feasible to reverse engineer an open egfp gene? Some point mutations would keep the sequence from being identical, no?--
Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC
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On Nov 22, 2012, at 4:15 PM, Cathal Garvey <cathal...@gmail.com> wrote:
> Patents. >:(
> Thanks. According to a ref on wikipedia, folding is impaired in the WT
> protein at 37C, so hopefully growing at 30C will help prevent that.
> Photostability is bad too, so will have to be sparing in my illumination
> of the samples..
>
> On 22/11/12 18:23, Josiah Zayner wrote:
>> That should work.
>> Any reason you used wtGFP and not one of the engineered versions?
>>
>> On Thursday, November 22, 2012 8:26:39 AM UTC-6, Cathal wrote:
>>>
>>> Hey all,
>>> I'm going to be working with a construct containing wildtype GFP, with
>>> the original peptide sequence from A.victoria, and I'm hoping someone
>>> here would know whether it's feasible to excite with Blue light (which I
>>> think you can do with normal GFP?) or if I'll have to use UV?
>>>
>>> Thanks
>>> Cathal
>
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