[DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

Happy $holidays!

A quick question: I need to sterile-filter several 10 l of a
reasonably viscous solution into autoclavable borosilicate
lab bottles.

This is typically done with syringe filters,

http://www.gilsonuk.com/catalogue/product/itemNo/ALG422A

but that's on a too small scale, and for rather nonviscous
solutions.

I was thinking about repurposing household items like
http://www.amazon.de/Keramikfilter-Wasserfilter-NEU-Filter-Feinfilter/dp/B002RLKE7O/
or http://www.amazon.de/Polypropylene-Auswaschbar-UMKEHROSMOSE-WASSERFILTER-SEDIMENTFILTER/dp/B002RLZS7U/

Has any of you done that? How did you sterilize the assembly?
(Polypropylene is obviously only cold-sterilizable, and
I suspect the plastic parts of the ceramics one won't
survive autoclaving either).

At this point I'm thinking about overnight soaking the ceramics
filter/whole filtering assembly in hydrogen carbonate/hydrogen
peroxide, 70% isopropanol or bleach, and flushing out the assembly
for an hour with reverse osmosis water, possibly prefiltered.

Thanks and a nice 2013 to ye all.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment