Alright this is a shot in the dark but if anyone can understand what I'm talking about and knows a company that sells custom stable lentiviral packaging cell lines (293t cells) which have already been transfected with some expression vector of a customers choosing please let me know. I know there are some that sell cell lines with lentiviral packaging vectors but can't find any that will transfect an expression vector in for me. The problem is that it's sorta easy to transfect cell lines will an expression vector and lentiviral packaging plasmids but this only results in transient lentiviral particle expression, that is to say the cells stop producing lentivirus particles after a few days. It is possible to make stable packaging cell lines it's just really hard (I can't do it on my own). I need a cell line that will produce lentivirus particles (which then can be used to integrate a gene of my choosing in other cells) for a long time (several months at least). So if anyone knows a company that sells or will make such a cell line please post a response.
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