Longer stretches of DNA are typically synthesized as shorter oligomers that are then stitched together.
The big differences lie in how much error correction and quality control is needed. When you're making oligos, you typically don't worry about having a small fraction of oligos with a single base error. However, when you're building longer constructs from error-prone oligos, you'll eventually get to the point where it becomes likely that every DNA strand you assembled contains one or more errors.
So you typically need to have much tighter control over how many errors get introduced at the oligo synthesis step, use methods for assembly that will automatically correct against errors, do QC at steps all along the process, and then sequence the resulting construct at the end to verify it is error free. That's a lot of overhead in addition to the oligo synthesis itself.
Patrik
On Monday, December 10, 2012 9:56:09 AM UTC-8, phillyj wrote:
On Sun, Dec 9, 2012 at 5:11 PM, Nathan McCorkle <nmz...@gmail.com> wrote:--
> Primers and probes aren't long DNAs though.
>
Is ordering a few long DNA more expensive than ordering many primers?
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