The idea is that dna is natures program. The code used to build the program is GCAT (Guanine
Cytosine Adenine Thyamine) - Which are nucleic acids. These nucleic acids are put in specific patterns
that eventually breakdown into specific proteins. When the proteins get expressed it gives you things
like eye color, hair texture, cell size, color of skin, etc..
In Biotech, you build your own programs using modified dna that you create. The programs(modified DNA)
you create, create proteins that do things like make plants glow.
In the lab (application of biotech) this is all easier to do than the complexity of theory may imply.
This is how you do it
How to Build a Program (modified DNA):
Get a Dna (extract from cell of organism you want to modify), Cut the Dna (using restriction enzymes), Insert some New DNA into the old Dna (by way of vector and dna ligase [glue] )
*you have to add a selection marker in the step where you would insert new dna.
the selection marker allows you to test the software to see if the program is running
How to Run the Program:
Clone the new Dna (Use PCR), Test to see if your new Dna is present. (by adding whatever chemical or environmental factor In order to activate the selection marker)
In this case, you will know that you successfully added the selection marker if your plant glows the color you want.
Of course there is more too it. Such as best practices of application, you also may need to know how exactly the
dna is run in the cell and more stuff. However, This is my attempt on giving you the basic info you need. You should also
google for a college lab that shows you exactly how to insert a gene into a bacteria - it will give you the exact materials they use in the lab. THESE MATERIALS ARE SOMETIMES MAD EXPENSIVE BUT THAT`S WHY DIYBIO IS IMPORTANT
P.S. - Listen bro, i'm new to this as well. But as long as you strive to see the big picture of every topic you come across you will always be able to figure out the intricate details, just keep the big picture in mind. Also time is
not an issue, trust me, if you are serious about learning, the passion will make every moment seem like not enough time. You will be feinding for more info every moment
Btw sorry for the last posts
On Wednesday, December 26, 2012 12:13:51 AM UTC-5, TJ Reece wrote:
Thank you Mega and Dakota for your input,The truth is, I only have a vague understanding of the theory behind what you're implying. And none whatsoever on the actual application process. I'm afraid I didn't emphasize enough on my rudimentary knowledge of biology in general. I understood prokaryotes to be single celled with no nucleus and eukaryotes to be the opposite(according to wikipedia this isn't always true). However I was able to google everything you wrote to get a common knowledge on the subject and am very thankul for your input. The question is now whether I would be able to pick up a few books and acquire the skill(s) related to do this, or if it's an accumulation of knowledge developed over years of practice?Btw, I live in central Pennsylvania, Harrisburg area.
On Tuesday, December 25, 2012 4:49:38 AM UTC-5, Mega wrote:Might I ask, where are you located?
Another addition:
For all light emitting reactions you basically need luciferin and luciferase which react to produce light.
You saw the thread about the pre-coelenterazine pepdide? This is a luciferin, and Renilla luciferase (which is broadly available) is the luciferase. So you could build a full light-generating pathway using just these two genes (adding two strong promoters). For comparison, the vbacterial lux operon needs LuxA+B (Luciferase) and Lux C+D+E+G, so in total 6 genes.
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