Re: [DIYbio] Hacking Stink Bugs

Greetings,

 

My 12 year-old daughter wants to be a molecular biologist. Sounds like quality time to me. I'm all in.

 

The project is RFLP of stink bug genomic DNA (Halyomorpha halys). We not looking towards cutting edge research. We'll be stoked if we can isolate, restrict, electrophorese and visualize.

 

I've collected some nice equipment including an electrophoresis system, centrifuge, pipettor, UV spectrophotometer and precision balance.  All that wasn't cheap but I really want this project to work because success will be inspirational to the lass.

 

Some questions to the community if I may?

 

I plan to use spin-column kits for DNA isolation.  In particular I like the EZNA kit from Omega Bio-tek.  Any comment on this choice?

 

Reagents are proving difficult to acquire.  I understand why suppliers frustrate the process.  But surely there is an avenue to quality reagents that is controlled yet accessible.  Any advice on how to inform suppliers?

 

Finally, as we are beginners, any other advice on getting started will be appreciated.

 

Best regards,

 

The Sticky Ends

Maryland, USA

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