Re: [DIYbio] Bacterial Mechanoreceptors

M. Pudica takes 5 days to sprout after a 24hr soak in hot water and 10 days till first contractile touch. Just keep it indoors under a standard desk light. If you want to get fancy keep it at 8000 lux with a 16hr photoperiod for optimal growth. It actually grows fast since it is a weed but needs warmth so indoors is ideal. The ion in question is Ca++ and it is stored in a separate tannin vacuole denoted by redish pigmentation near the base or the leaf and intersection of the branch to the main stem. Just FYI.

Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC

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On Dec 21, 2012, at 8:39 AM, Cathal Garvey <cathalgarvey@gmail.com> wrote:

> This mechanism matches well with that used by Dionaea muscipula (Venus
> Flytrap): the closing of the traps is accomplished by expansion of the
> outer layer of the trap/leaf tissue, not by contraction of the inner
> layer. Unlike Mimosa pudica it's a one-way issue, apparently traps are
> usually only good for 3-6 rounds of trapping, but they get bigger with
> each cycle of opening and closing.
>
> And, like M.pudica, it's an ion/osmosis influx mechanism, though I read
> about it in a dead-tree book so long ago that I can't provide a citation
> or even a guarantee of which ion is involved.
>
> In other news, I actually have seeds for M.pudica, but never bothered
> sprouting them; I've heard it's a really annoying plant to cultivate and
> takes ages to grow. A friend has one, and I've poked it for fun; very
> satisfying! But, according to her it's languishing in the Irish climate
> and probably won't survive another year. :-/
>
> On 19/12/12 00:02, Sebastian S. Cocioba wrote:
>> My original goal was to try to cassette the mechanoreceptor genes from mimosa pudica but the issue is that the system in question is A). Poorly understood, and B) relies on turgor pressure loss of specific cells called pulvini motor cells. It involves very specific tissues acting like hinges and once hydraulic pressure lost the leaf or branch moves. I think dinos are the way to go. From what I know the dinos respond to bumping by hair like triggers on their surface. I am looking for a simple structure that will change conformation once touched or bumped and induce some gene expression down the line that can be exploited into a controllable switch with gfp expression as its verification packet. Thanks Nathan for the tip. Will keep posted on any findings.
>>
>> Sebastian S Cocioba
>> CEO & Founder
>> New York Botanics, LLC
>>
>> Sent via Mobile E-Mail
>>
>> On Dec 18, 2012, at 1:53 PM, Graeme Dean <itisgraemedean@gmail.com> wrote:
>>
>>> Hi, Just wondering, If you are going to try making something tap sensitive have you thought about working with something like Mimosa pudica? I have done a small amount of looking and have not found much, I would be very curious about anything people have to say or have found/are doing on this subject.
>>>
>>> On 18 December 2012 18:24, Nathan McCorkle <nmz787@gmail.com> wrote:
>>>> I'd look into the dinoflagelletes as they seem to already do the 'bump and glow' thing (they're closer to your plants too)
>>>>
>>>>
>>>> On Mon, Dec 17, 2012 at 2:33 PM, Sebastian S. Cocioba <scocioba@gmail.com> wrote:
>>>>> In the process of studying rapid movement in plants, I stumbled upon a large research field in the topic of mechanosensitive ion channels in bacteria. The concepts between bacterial and plant shock sensing is similar in that they both release or take in an ion as a secondary messenger as a result said shock. I am curious if any DIY Bio or iGEM ppl worked with this concept and, lets say, tie it to gfp expression so one could make a tap sensitive glow under UV? A genetic momentary switch of sorts. Just curious...
>>>>>
>>>>> Sebastian S Cocioba
>>>>> CEO & Founder
>>>>> New York Botanics, LLC
>>>>>
>>>>> Sent via Mobile E-Mail
>>>>>
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>>>>
>>>>
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