Thanks for the response Matt, makes sense. Now that you mention it, I had forgot that when I programmed the old peltier cycler, I did repeat step 1 30 times, when I think I should have done 29 times, to make it 30 in total. So...I think the peltier machine actually did 31 cycles, not 30, so it could be the reason for the brighter bands in the peltier lanes.
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