I think you're looking at more than a two fold difference in yield. Perhaps 10 - 50 fold more. Suggests that the Peltier is more efficient ( meaning better extension, doubling of more cycles). May be the trade off from the rapid cycling with the Idaho machine. The average Taq polymerase will extend 1 kb target in about a minute .... so maybe the short 45 second extension at 70 - 72 is penalizing yield in the rapidcycler? Less of an issue with the shorter first target & primers.
Room to play a little to optimize yields with the rapidcycler.
>matt
Sent from my Verizon Wireless 4G LTE DROID
Room to play a little to optimize yields with the rapidcycler.
>matt
Sent from my Verizon Wireless 4G LTE DROID
-----Original message-----
From: Dakota <dkotes@gmail.com>
To: "diybio@googlegroups.com" <diybio@googlegroups.com>
Sent: Tue, Dec 25, 2012 14:26:59 CST
Subject: Re: [DIYbio] First full DNA extraction, PCR, and gelThanks for the response Matt, makes sense. Now that you mention it, I had forgot that when I programmed the old peltier cycler, I did repeat step 1 30 times, when I think I should have done 29 times, to make it 30 in total. So...I think the peltier machine actually did 31 cycles, not 30, so it could be the reason for the brighter bands in the peltier lanes. --
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