Re: [DIYbio] First full DNA extraction, PCR, and gel

Yeah some optimization is definitely in order, but it should be enough DNA to sequence at least.  The Idaho machine is designed to use glass capillary tubes, but ours came with a tray which I believe is for thin walled normal PCR tubes.  The capillary tubes specific for this machine are discontinued, and the company that makes them, Bio-Fire or something, said they may or may not have them in stock.  Though I can probably get my hands on some, and loading them won't be a problem with capillary action, the after process seems to be a bit of a pain.


You have to flame seal them like an ampule, which removes the need for mineral oil, but is an added step, and then to remove the sample from the tubes you need some special needle aspirator or tiny pipette thing that costs $100 + who knows how much for tips.

When I was reading about the rapid cycler system, they said that you could do 1kb or less product amplifications with something like a 0 or 1 second denature and anneal time, and a 10-15 second extension time, if you use the glass capillaries.  


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