Re: [DIYbio] First full DNA extraction, PCR, and gel

Yeah I was pretty impressed with it, it did a great job for being $50 off ebay.  I havn't really looked at heating ramp times compared to peltier, but when the lid in the top of the machine opens, it cools very fast.  The only downsides I can think of quickly is the need for mineral oil as well as not being able to do a 4 degree C final hold.


But, if you can give up those two things, it saves a lot of time.  After we get the sample sequenced, going to re-do some amplifications and drop extension and annealing times by  5s or 10s a run and still see if PCR works. 

I re-did the write-up and posted it, http://basementbiotech.org/  but it's basically the same, with just an extra picture of two.

I'll be one of the first in line if AirPCR comes out!

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