Enzymes are routinely stored in a 50% glycerol solution at -20C. They do not freeze because of the glycerol. Freeze / thaw would likely kill or seriously reduce enzyme activity. All these things are enzyme specific. Some enzymes don't like low temps or glycerol, but this is relatively rare. If these enzymes are sold commercially, check if anyone sells them as a glycerol stock.
Jim Lund
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