I tried this myself as well, because I have never preformed electrophoresis (agarose in the mail!), I thought it might be easier for my to pick up. I will post pictures when I am done.
Basically I put some loading die on a straight line against some normal printer paper. A microscope slide blocked it from going through the rest of the paper. Then I dipped it with only the bottom slide on into some TBE buffer. Once it was soaked, I put the top on, then I left it inside the electrophoresis apparatus at 50v (Since I want to go to sleep soon)I will check it every thirty minutes.
-Koeng
On Saturday, January 19, 2013 4:11:43 PM UTC-8, Nathan McCorkle wrote:
So my thinking is everyone can get paper of some form, but not--
agar/agarose... seems it's not ideal for DNA but it is for amino acids
and small proteins... it might be useful for DNA or large proteins in
certain assays though... i.e. yes/no assays or something
here are some things i dug up:
mentions a few paragraphs of history
https://www.idtdna.com/pages/docs/educational-resources/ gel-electrophoresis.pdf
including
"
While paper and other solid support materials proved to be an
advantage over free
solutions for the electrophoretic analysis of biomolecules, gels were
adopted later
because gels not only minimized diffusion better than paper supports
they actually
participated in the separation process by interacting with the
migrating particles.
"
and referencing this article about Bothrops (pit viper snake) venom,
but it's in German (I think) and I can't read it (google translate
doesn't do much either because copy-paste don't keep the sepcial
characters)
http://nathanmccorkle.com/pdf/this%20might%20be%20about% 20paper%20electrophoresis.pdf
I got thinking because I saw this chromatography paper for $0.65 per
foot and mentions "widely used for electrophoresis"
http://store01.prostores.com/servlet/thescienceshop/the- 2012/CHROMATOGRAPHY-PAPER-2cm- Wide/Detail
thesis on
"Development of Paper electrophoresis technique for observation of
microgram quantities of protein" and "electrophoretic and
ultracentrifugal studies of soluble antigen-antibody complexes as a
method of determining antibody and antigen valences"
James T. Bradbury, California Institute of Technology, 1956
http://thesis.library.caltech.edu/995/1/Bradbury_jt_1956.pdf
--
-Nathan
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