So I cut out a slide sized piece of coffee filter paper (sadly unbleached and lightly brown) wrapped it with two strips of parafilm but made sure the narrower edges were exposed. I dispensed 10uL at the negative pole edge near the center of the opening. It dispersed about a half inch down the paper slide sandwich. I made two samples and put them in my tiny electrophoresis chamber at 25v. A normal 1% gel takes 3 hours at this setting so im gonna let it run for that long. Ill check every hour and will post on imagur once its done.
Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC
Sent via Mobile E-Mail
I did a quick run with marker dyes 10 minutes ago as I didn't have any DNA or food coloring. Tried it once with 1x TBE and once with salt water. Once without the glass sandwich and once without the glass sandwich. The first picture was just to make sure the dyes dispersed in all directions without a voltage applied.When I ran them I completed the battery circuit, sometimes using the automotive bulb as a resistor to keep current @ no more than 400mA. I tried using my gel box supply but it was giving leak load errors and other BS so I didn't even bother.Basically...the results were completely useless since I never had a good control, and the one time it looked like the dyes were migrating, the slide was slanted and gravity had its way with them. I was also impatient and didn't wait very long.BUT. I can say the glass slide sandwich is much nicer to deal with, and capillary action inside it allows for the electrolyte to quickly meet in the middle. I dotted in the middle and then dropped electrolyte at the lip on both the + and - ends and they met in the middle.With DNA, you could assume it'd be moving away from - towards positive, but I didn't know the charges of the dyes off hand so starting in the middle seemed best.Anyway, pretty useless but it looks pretty. I'll be interested to see how Sebastian's run comes out. I'll try again later if I can get the gel box to work. I assume 9V was not enough to make them run fast.GO TEAM--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment