Re: [DIYbio] electrophoresis... path length design strategies (was: Paper electrophoresis... . . .)

http://imgur.com/a/t1SEv


Re-doing the experiment but with coffee filter paper, and a real gel box, and a real power supply.  As you can tell from the first picture, the chem filter paper I have allows the dye to disperse way too fast, as it should, since it's supposed to let solvents pass through it quickly.  Sebastian seemed to have decent sounding results using the brown coffee filter paper, so we'll see how it works with the dyes.

I tried to reduce pouring turbulence too much when pouring in the buffer but, was unavoidable when the solvent front rushed through the slide sandwhiches.  Running at 120V 100mA or there abouts.

There is already noticeable migration of one of the components of the markers to one direction---anyone fancy a guess?  You'll win the Marker Migration badge of honor.

I'll try to post a video later if you want to experience the most intense marker dye coffee filter electrophoresis you've ever seen.  Blow your mind if it was in 3D.

GO TEAM

Oh and the candles weren't to set the mood, was going to try that dipping strategy to section off a running lane and prevent bleeding.  Maybe next time

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment