I see where you are going with the slide sandwich idea. I have some spare ladder and will run it like you said in my gel box. Parafilm along the edges might work, no? Im really curious to see how my insta-stain etbr stickers will work on the paper...I'll have my results in 3hrs. Ill run the paper at low voltage since my box is tiny (gibco horizon 48 @ 25v at IDK milliamps).
Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC
Sent via Mobile E-Mail
On Jan 29, 2013, at 6:06 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> On Tue, Jan 29, 2013 at 2:03 PM, Sebastian Cocioba <scocioba@gmail.com> wrote:
>> Ill run one when I get home too. How should we dispense the dna onto the
>> paper? Do you think printer paper left to soak for a while would work?
>> Tissue paper? Whatman? I fear the sheer density of any papet would result in
>> low transit.
>
>
> Maybe spot the DNA TLC-style on dry paper, sandwich that between two
> microscope slides, seal the long edges with wax immerse... then put
> the glass sandwich between two buffer tanks containing the electrodes.
> You'd probably need to make gaskets for either end of the glass
> sandwich though.
>
> Any other ideas? I feel like you need glass plates because I think it
> would restrict the DNA from diffusing into solution.
>
> --
> -Nathan
>
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Re: [DIYbio] Paper electrophoresis... super available molecule separation?
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