What may be feasible (but labour intensive): Make the cell produce the antidote with nuclear localization signal and toxin with chloroplast transit peptide and kanamycin resistance (for cell selection!).
At the same time you'd have to transform the chloroplasts with the genes of interest and the resistance.
On Thu, Jan 31, 2013 at 10:55 PM, Andreas Sturm <masterstorm123@gmail.com> wrote:
Thanks for that Nathan!
Now I have the sequences of biosynthesis pathway for kanamycin and streptomycin antibiotics ;)
However, both are kind of big. KanSynth ~ 10 kbp ; strepto Synth ~ 18 kbp.
The toxin/antitoxin has just some <1kbp in total!!
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