DNA Cleanup kits not required for straight PCR; rarely necessary in fact.
You ought to be very careful buying PCR machines on Ebay; they are often only tested to turn on, but if there's trouble, the trouble sets in when the machine heats or cools.
Given the shipping costs of getting such a giant heat-sink sent to you for proper testing, you can end up screwing yourself on second-hand equipment.
There's an old argument on the lists about this issue, but I for one suggest supporting community-designed, built and managed hardware that you can rely on community support for. The best available hardware fitting this description is the OpenPCR.
Of course, if cost is an issue, you can always build your own! :)
On 4 January 2013 21:17, Josiah Zayner <josiah.zayner@gmail.com> wrote:
The problem isn't really the cost per run it is the initial investment of buying primers, DNA extraction kits, polymerase, DNA clean up kits.
Initial investment is probably around $400 not including sequencing or the PCR machine.
Buy a PCR machine on ebay and save yourself $400+.To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/BnCrWf9Tj7oJ.
On Monday, December 24, 2012 6:56:24 PM UTC-6, jcrubino wrote:What is the cost per run on the openPCR or costs associated with operating the openPCR?--Looking to explore the family tree if possible.ThanksJR
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