Re: [DIYbio] Re: Paper electrophoresis... super available molecule separation?

Sadly I let my Kombucha stale in the fridge for too long and couldn't
recover it. And, my isolated culture of G.xylinus is long dried out too,
due to a gap in the parafilm. So, I'll have to re-order Kombucha and
re-isolate G.xylinus! Thankfully, the latter isn't too challenging.

You make agar containing calcium carbonate powder, stir and pour right
before solidifying so you get evenly dispersed CaCO4 particles through
the gel, then streak and re-streak your kombucha on the surface. The
colonies that create a broad area of clear agar (acid dissolving the
CaC04) are your Geo/Acetobacter, of which there may be one or two species.

Isolate those and grow to stationary, the plates that form nodules of
whitish cellulose after a week or so are your guys. :)

I have the agar recipe lying around somewhere, will dig up soon.

On 30/01/13 06:06, Patrik D'haeseleer wrote:
> Hey Cathal - do you have some Kombucha going right now? I'd love to see
> what happens if you take a nice thick "mother" SCOBY, cut some wells
> into it, and use it as an electrophoresis gel. :-). Food dyes
> electrophorese pretty well.
>
> Patrik
>
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