Re: [DIYbio] Re: Paper electrophoresis... super available molecule separation?

The "mesh strength" of your gel/paper is also relevant, so if you're
using dense paper you would expect slower migration. Given that paper
varies very widely in quality, density and chemical purity, you'd
probably have to work the protocol to the brand of paper you're using
and try to stick to the same brand.

I wonder how big a difference it would make to use archival-grade paper,
which tends to have less bleaching byproducts/acids, less tannin, and
often higher-quality cellulose ~= longer cellulose fibers. Probably
you'd get a more even matrix of cellulose leading to more predictable
and even migration of bands, if it worked at all?

On 30/01/13 17:45, Nathan McCorkle wrote:
> On Wed, Jan 30, 2013 at 9:35 AM, Jeswin <phillyj101@gmail.com> wrote:
>> On Wed, Jan 30, 2013 at 8:48 AM, Dakota Hamill <dkotes@gmail.com> wrote:
>>>
>>> Trying to think of other ways to seal off a narrow channel, to control the
>>> initial dispersion as you said, but keep open a running lane for the DNA or
>>> dye to migrate.
>>>
>>
>> I don't think we should think of paper electrophoresis in relation to
>> gel electrophoresis. For example, in gel electrophoresis, we run
>> multiple lanes at once. For paper, maybe it's best to run 1 sample per
>> strip. That will prevent the diffused molecules from merging together.
>> Multiple strips of the same length can probably be run together and
>> still be good for comparision. The voltage through all the strips
>> should be the same and so should the rate of migration. Correct me on
>> this last statement.
>
> I think the distance between the electrodes makes the most impact
>
>

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